ご注文情報
| 製品 | 包装 | コード番号 | 価格（円） | ||||
|---|---|---|---|---|---|---|---|
| Handbook Antibody Purification （英文、2007年発行） | 1 冊 | 18103746 | 販売終了 | ||||
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詳細情報
項目
- 目次
Introduction
Chapter 1. Antibody structure, classification, and production
Native sources
Genetically engineered sources
References
Chapter 2. Sample preparation
Sources and their associated contaminants
Extraction of recombinant antibodies and antibody fragments
Clarification of serum, ascites, cell culture supernatant, or cell lysate
Sample preparation before purification
Desalting and buffer exchange
Chapter 3. Small-scale purification by affinity chromatography
General considerations
Adding a polishing step after intitial purification
Protein G and protein A bind to different IgG
Purification using Protein G Sepharose™ media
Purifying Fab and F(ab')2 fragments
Multiwell plates and columns prepacked with Protein G Sepharose™ High Performance
Purification using protein A-based media
MabSelect™ media and prepacked columns
Purification of other classes of antibodies
Making immunospecific purification media with custom ligands
Chapter 4. Removal of specific contaminants after initial purification
Bovine immunoglobulins
Albumin and transferrin
a2-macroglobulin and haptoglobulin
Dimers and aggregates
DNA and endotoxins
Affinity ligands
Chapter 5. Automated purification of antibodies using ÄKTA™design systems
Chapter 6. Multistep purification strategies
Examples of multistep purification
Chapter 7. Large-scale purification
Platform technologies in MAb purification
Process design
High productivity media for MAb purification
Prepacked, disposable solutions speed up the downstream process
Custom Designed Media and columns
Appendix
Chapter 1. Antibody structure, classification, and production
Native sources
Genetically engineered sources
References
Chapter 2. Sample preparation
Sources and their associated contaminants
Extraction of recombinant antibodies and antibody fragments
Clarification of serum, ascites, cell culture supernatant, or cell lysate
Sample preparation before purification
Desalting and buffer exchange
Chapter 3. Small-scale purification by affinity chromatography
General considerations
Adding a polishing step after intitial purification
Protein G and protein A bind to different IgG
Purification using Protein G Sepharose™ media
Purifying Fab and F(ab')2 fragments
Multiwell plates and columns prepacked with Protein G Sepharose™ High Performance
Purification using protein A-based media
MabSelect™ media and prepacked columns
Purification of other classes of antibodies
Making immunospecific purification media with custom ligands
Chapter 4. Removal of specific contaminants after initial purification
Bovine immunoglobulins
Albumin and transferrin
a2-macroglobulin and haptoglobulin
Dimers and aggregates
DNA and endotoxins
Affinity ligands
Chapter 5. Automated purification of antibodies using ÄKTA™design systems
Chapter 6. Multistep purification strategies
Examples of multistep purification
Chapter 7. Large-scale purification
Platform technologies in MAb purification
Process design
High productivity media for MAb purification
Prepacked, disposable solutions speed up the downstream process
Custom Designed Media and columns
Appendix
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